Spatial Transcriptomics

Map the whole transcriptome within the spatial context of the tissue to uncover novel insights and discover biomarkers

*For Research Use Only (not for use in diagnostic procedures)

What is Spatial Transcriptomics?

Spatial relationships between cells and structures are critical to their development and pathophysiology. With the emergence of spatial transcriptomics technologies, we can now bridge histology and genomics to deepen our understanding of cellular organization and gene expression within a tissue of interest.

With our 10X Genomics Visium CytAssist workflow, the whole transcriptome is mapped within the histological context of the tissue using either formalin fixed paraffin embedded (FFPE) blocks or pre-sectioned tissues on glass slides as starting material, giving you the ultimate flexibility.

We provide a complete seamless package of end-to-end wet lab to dry lab services meaning we can take care of everything from sample processing to data analysis enabling you to gain valuable, reproducible, high-quality insights. All we need are formalin fixed paraffin embedded (FFPE) tissue blocks or tissue sections (stained or unstained) on glass slides and we will take care of the rest.

Our Visium CytAssist Process
Key Benefits of Spatial Transcriptomics
We Make Spatial Transcriptomics Look Easy
Deriving Novel Insights to Accelerate Your Research

Our Visium CytAssist Process

Visium CytAssist Process at AcelaBio

Bringing you high-precision Spatial Transcriptomics in an end-to-end workflow that accelerates your research. We make your study our priority with a dedicated project manager delivering regular study updates ensuring a seamless communication process from start to finish

Hover over the icons below to check out each step of our seamless end-to-end workflow:

Study Design Consultation

Study Design Consultation

Sample Collection and Processing

Sample Collection and Processing

Quality Control and Tissue Imaging

Quality Control and Tissue Imaging

Decrosslinking and Probe Hybridization

Decrosslinking and Probe Hybridization

Analyte Transfer

Analyte Transfer

Probe Extension and Library Construction

Probe Extension and Library Construction



Data Analysis and Interpretation

Data Analysis and Interpretation

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Key Benefits of Spatial Transcriptomics at AcelaBio

At the forefront of science and innovation, AcelaBio is one of the first laboratories to offer a complete end-to-end spatial transcriptomics workflow leveraging 10X Genomics Visium CytAssist. Our seamless, high-quality process from wet lab to dry lab translates your samples into meaningful biomarker insights.

Sample Flexibility

Analyze formalin-fixed paraffin-embedded (FFPE) tissue blocks or tissue sections from human or mouse samples.

Full Tissue Coverage

Capture more data by analyzing the whole tissue section with high sensitivity. Choose reaction areas of 6.5 x 6.5 mm to 11 x 11 mm, avoiding the need to limit analysis to small regions of interest.

High Cellular Resolution

Interrogate gene activity at an average resolution of 1-10 cells per barcoded spot on the 10X Visium tissue slide.

High Quality, Reproducible Results

Molecular pathology workflow integrates seamlessly with standardized and validated histology workflows.

In-depth Data Analysis & Interpretation

Our stepwise bioinformatics approach assesses quality and analyzes spatially resolved biomarkers, signaling pathways, and cell populations.

Biomarker Discovery to Development

Once biomarker(s) are identified, targeted assays can be developed, allowing for high throughput analysis.

Unlock the potential of your tissue sample

The key to the 10X Visium Spatial Gene Expression solution is the Visium CytAssist Spatial Slide with capture areas of 6.5 x 6.5 mm or 11 x 11 mm. For 6.5 mm2, each of its two capture areas includes ~5,000 barcoded spots, for 11 mm2, each of its two capture areas includes ~14,000 barcoded spots, which in turn contain millions of spatially barcoded capture oligonucleotides. Upon permeabilization, tissue mRNA is released and attaches to the barcoded oligos, enabling the collection of gene expression data.

From early-stage discovery to clinical research, spatial transcriptomics can unravel novel insights and accelerate biomarker discovery within the histological context.

We Make Spatial Transcriptomics Look Easy

Define and visualize tissue architectures from spatially resolved transcriptomics with AcelaBio.

Our experienced scientific team provides seamless delivery from project initiation to delivery of data.

One-Stop-Shop for All Your Needs

Save time and resources by accessing our end-to-end spatial transcriptomics service

High Quality

Our workflows are optimized and validated in line with clinical quality standards

Interactive Data Analysis

We work closely with you to achieve the level of analysis you require to derive meaningful discoveries

Long-Lasting Partnership

We provide solutions throughout the entire lifecycle of product development – from biomarker discovery to biomarker development


We can develop different orthogonal methods (e.g., single- or multiplex-immunohistochemistry) to validate targets discovered through the spatial transcriptomics workflow

End-to-End Spatial Transcriptomics Service

Learn More about our Visium CytAssist Process

Deriving Novel Insights to Accelerate Your Research

Spatial transcriptomics generates ‘big’ data requiring programming and statistical expertise to maximize biomarker discovery

We are serious about data analysis

Our analysis and visualization packages go beyond the standard assessments and dive deep into the data to increase the value of your output. We offer a flexible, iterative three-step approach based on your study objectives with the goal of helping you understand what your data means through integration with highly curated datasets.

Quality is key. Our first step is to pre-process the data and evaluate quality metrics to ensure the data meets our quality standards and is ready for analysis in our downstream bioinformatics pipelines. Sequencing data will be aligned to the appropriate reference transcriptome, mapped to the histopathology image and gene counts will be determined using the 10X Genomics recommended Space Ranger pipeline.

Key Deliverables:

  • Tissue positions
  • Scale factors
  • Image outputs
  • Feature-Barcode Matrices
  • QC report

To explore the spatial gene expression data, our analysis pipeline is designed to classify the data into clusters and annotated histological features, explore cluster proportions per experimental condition, and find spatially-resolved biomarkers.

Key Deliverables:

  • Clustering UMAP and Spatial graphs
  • Cluster proportions graph and table
  • Gene markers heatmap and table
  • Gene markers spatial heatmap

Once you know what genes are active and where the activity is located in the tissue, the key question is, what does your data mean? We provide context to your results by integrating your data with high-quality curated datasets of biomarkers, cell types and signaling pathways to better understand the cellular heterogeneity, annotate cell subtypes, identify significantly enriched signaling pathways, and map pathway activity into the histology slides. As we unravel novel insights, we will work iteratively with you to achieve the depth of analysis that meets your needs.

Key Deliverables:

  • Identification of enriched signaling pathways by Gene Set Enrichment Analysis (GSEA)
  • Mapping pathway activity into the histology slides
  • Identification of cell composition

Partner with a 10X Certified Service Provider

As one of the earliest adopters of 10X Genomics’ Visium CytAssist technology, we pride ourselves on being a trusted spatial transcriptomics partner to leading pharmaceutical and biotechnology companies. AcelaBio is a 10X Certified Provider and has been recognized by 10X Genomics for its expert ability to deliver high quality, reproducible spatial transcriptomics data in a timely manner.

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What tissue types do we work with?

When submitting FFPE tissue, the Visium Spatial Gene Expression solution is available for human and mouse tissue samples. We can work with blocks, or tissue sections affixed to glass slides (stained or unstained). For the complete list of tested tissue, please refer to the 10X Genomics website.

What is the size of the spots on the Visium Spatial Slide?

Spots are 55 µm in diameter.

Does Visium enable single cell resolution?

The current Visium solution has barcoded spots that are 55 µm in diameter which captures approximately 10 cells. Through our bioinformatics approach, we can perform data deconvolution to analyze data at the cellular level.

What is the configuration of the capture area of the Visium Spatial Slide?

The capture area is 6.5mm x 6.5mm or 11mm x 11mm.

*For Research Use Only (not for use in diagnostic procedures)
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