Services

Cross-referencing of sample identifiers with shipping manifest or requisition form

We provide end-to-end histopathology and precision medicine services from sample to score for clinical trials and clinical research.

 

AcelaBio’s pathology workflow is fully digital and includes automated sample tracking with a documented audit trail from sample receipt to sign-out. Our team brings expertise in providing clinical research services to pharmaceutical, biotech, and academic institutions.

Accessioning

Accessioning

Cross-referencing of sample identifiers with shipping manifest or requisition form

Confirmation of completeness of sample-associated meta-data

Registration of sample in laboratory information system

Processing

Processing

Fully automated process to dehydrate, clear, and infiltrate the tissue

Embedding

Embedding

Embedding of processed tissue into a wax block

Ensuring that tissue is oriented in a correct way for downstream microtomy

Sectioning

Sectioning

Microtomy with sectioning of the wax block to cut sections of typically 4 µm thickness

Tissue sections are subsequently placed on positively charged glass slides

Hematoxylin & Eosin

Hematoxylin & Eosin

Automated staining of tissue on a glass slide with hematoxylin and eosin

Hematoxylin stains cell nuclei a purplish blue

Eosin stains the extracellular matrix and cytoplasm pink

Special Stains

Special Stains

Automated special staining of tissue on a glass slide, such as with Masson Trichrome

Immunostaining

Immunostaining

Automated staining with antibodies linked to chromogens to identify a certain target

Single or multiplex chromogenic immunohistochemistry (IHC)

Assay Development

Assay Development

Development of a new staining protocol, typically using immunohistochemistry

The starting point can be a known target, an antibody, or an in vitro diagnostic (IVD)

Antibody Optimization and Validation

Antibody Optimization and Validation

Process of characterizing and validating antibodies for the identification of biomarker targets

Testing out different dilutions, buffers, and reaction kits

Whole Slide Image Scanning

Whole Slide Image Scanning

Digital pathology using 510(k) approved scanner to scan whole glass slides up to 40X

Digitized slides can then be transferred to an image management system for pathologist review

Pathology Review

Pathology Review

Support assay development as it relates to ensuring staining pattern is correct

Identification and annotation of tissue areas, structures, and cells to support the development of algorithms for quantitative digital image analysis

Review of clinical cases, which may include disease activity scoring and diagnosis

Data Management

Data Management

Delivery of consistent data & imagery through automated laboratory information systems

Sample Logistics

Sample Logistics

Preparation and shipment of blocks and slides to national and international locations

Storage & Archiving

Storage & Archiving

Semi-automated archival system of blocks and slides in temperature & humidity-controlled environment

Consulting

Consulting

Implementation of histopathology services in multicenter, global clinical trials

Selection of biomarker targets based on mechanism of action and technical capabilities

Project Management

Project Management

Operational experts ensure projects are delivered on time within budget

In Situ Hybridization (ISH)

In Situ Hybridization (ISH)

Use of specific probes to identify target gene transcripts in tissue using RNAscope

Clinical Trial Support

Clinical Trial Support

AcelaBio works in partnership with Alimentiv, a leading full-service niche CRO specialized in gastroenterology, to provide an end-to-end clinical study management solution that ranges from early trial design to complete execution of a clinical trial.

**For GI Services Only

Digital Image Analysis*

Digital Image Analysis*

Quantitative digital image analysis using machine learning and AI techniques

Custom algorithm development with oversight of expert pathologists

Generate quantitative, reproducible data on whole slide images or specific regions of interest

*For Research Use Only (not for use in diagnostic procedures)

RNA Extraction*

RNA Extraction*

Extraction of the whole RNA from formalin-fixed paraffin-embedded (FFPE) tissue blocks or sections

Allows for downstream bulk RNAseq and generation of insights at the gene transcript level

*For Research Use Only (not for use in diagnostic procedures)

Spatial Transcriptomics*

Spatial Transcriptomics*

Mapping of the whole transcriptome with morphological context using 10X Genomics Visium

Ability to analyze existing formalin-fixed paraffin-embedded (FFPE) tissue blocks or sections

*For Research Use Only (not for use in diagnostic procedures)

Bioinformatics*

Bioinformatics*

Leverage existing pipelines for RNAseq data analysis, including primary analysis, QC, differential gene expression analysis, and pathway analysis

*For Research Use Only (not for use in diagnostic procedures)